HPLC of Morphine with Electrochemical Detection: Analysis in Human Plasma
نویسندگان
چکیده
منابع مشابه
Analytical method of measuring tea catechins in human plasma by solid-phase extraction and HPLC with electrochemical detection.
We developed an analytical method for measuring tea catechins in plasma by solid-phase extraction (SPE), followed by HPLC with a coulometric electrochemical detector. The plasma was mixed with an equal volume of acetonitrile to precipitate protein, and catechins in the resulting supernatant were extracted by SPE, using a C18 cartridge. To correct the extraction efficiency, ethyl gallate was sim...
متن کاملDetermination of codeine and morphine in human plasma by high performance liquid chromatography with serial electrochemical detection.
A method is described for the determination of codeine and its metabolite, morphine, at low nanogram concentrations in plasma. Analysis is accomplished by high-performance liquid chromatography utilizing a cyanopropyl normal bonded phase (NBP) column in reversed-phase mode and two electrochemical detectors in series configuration. Two internal standards are utilized, ethyl morphine for codeine ...
متن کاملApplication of HPLC Analysis of Medroxyprogesterone Acetate in Human Plasma
Generally, progestogens, i.e. progesterone in combination with oestradiol esters, are used primarily in the treatment of menstrual irregularities to maintain endangered pregnancies. Their derivatives are orally active medroxyprogesterone acetate including hydroxyprogesterone caproate, chlormadinone acetate, megestrol acetate. Naturally occurring glucocorticoids are often used in medicine such a...
متن کاملHPLC-UV determination of morphine in human plasma and its application to the clinical study.
A sensitive and specific high-performance liquid chromatography method with ultraviolet detection (HPLC-UV) has been developed for the quantification of morphine sulfate [(5alpha,6alpha)-7,8-didehydro-4,5-epoxy-17-methylmorphinan-3,6-diol], (CAS: 52-26-6) in human plasma. The analyte was extracted from plasma samples with chloroform - isopropyl alcohol (90:10, v/v) and analyzed on a Bondapak C1...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
سال: 1984
ISSN: 0004-5632,1758-1001
DOI: 10.1177/000456328402100209